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ACMG technical standards and guidelines: microarray analysis for chromosome abnormalities in neoplastic disorders (2013) (view source)
Revision as of 16:25, 27 July 2016
, 16:25, 27 July 2016→Definitions
Dilution studies for SNP arrays require nonneoplastic and tumor DNA from the same patient. Buccal cells or blood may provide a source of nonneoplastic patient DNA.
Dilution studies for SNP arrays require nonneoplastic and tumor DNA from the same patient. Buccal cells or blood may provide a source of nonneoplastic patient DNA.
Assessment of levels of neoplastic to nonneoplastic cells or sizes of different clonal populations in fresh or fixed (FFPE) tissue samples is more difficult. Dissection of fresh tumor with an inverted microscope can reduce the amount of nonneoplastic tissues. Microdissection of FFPE tumors can enrich the DNA sample for tumor. Estimation of clonality in tumor tissue samples can be useful when analyzing data from these tumor types.<ref name=11></ref><ref name=Nowak></ref>
Assessment of levels of neoplastic to nonneoplastic cells or sizes of different clonal populations in fresh or fixed (FFPE) tissue samples is more difficult. Dissection of fresh tumor with an inverted microscope can reduce the amount of nonneoplastic tissues. Microdissection of FFPE tumors can enrich the DNA sample for tumor. Estimation of clonality in tumor tissue samples can be useful when analyzing data from these tumor types.<ref name=Slovak></ref><ref name=Nowak></ref>
Determination of ploidy
Determination of ploidy